Methyl jasmonate effects on Lactuca serriola L.: Antioxidant defense and bioactive compound changes

MeJA effects on Lactuca serriola

Authors

  • Maisa Asheri PhD student
  • Alireza Farokhzad Associate professor of Department of Horticultural science, Faculty of Agriculture, Urmia University, Iran
  • Mohammad Naghavi Professor of Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran
  • Raheleh Ghasemzadeh Assistant professor of Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Iran
  • Pejman Azadi Associate professor of Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
  • Meisam Zargar Associate professor of Department of Agrobiotechnology, Institute of Agriculture, RUDN University, 117198 Moscow, Russia

Keywords:

Antioxidant enzymes, prickly lettuce, total flavonoid, total phenol

Abstract

The effect of methyl jasmonate (MeJA) foliar spray on the activity of antioxidant enzymes—Superoxide dismutase (SOD), Catalase (CAT), Ascorbate peroxidase (APX), and Guaiacol peroxidase (GPX)—along with assessments of total phenolic and flavonoid contents and antioxidant activity (IC50), was examined in Prickly lettuce (Lactuca serriola L.). The study involved treating plants with three MeJA solutions (0, 200, and 400 µM) and harvesting samples at four distinct time intervals. Varied MeJA concentrations and time intervals resulted in a substantial increase in the activity of all the antioxidant enzymes investigated in this study. Both concentration levels and time courses exhibited progressive outcomes. Moreover, MeJA treatment led to elevated levels of total phenolic and flavonoid contents, reaching peaks of 17.02 (mg GAL/g DW) and 8.3 (mg QUE/g DW), respectively, particularly in response to the 400 µM concentration. However, the total flavonoid content did not show any significant variation between the two concentrations. Based on the half-maximal inhibitory concentration (IC50) values, the antioxidant activity in MeJA-treated plants was found to be lower compared to the controls. However, our findings suggest that, under specific conditions discussed in this study, MeJA has the potential to enhance the nutritional value of L. serriola.

Published

2024-10-31

Issue

Section

Original Research Articles