Clinicopathological characteristics of SMAD4 gene expressions in colorectal cancer patients

SMAD4 gene and colorectal cancer

Authors

  • Neda Mansouri Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca). IBSAL and CIBERONC, Salamanca, Spain
  • Zahra Mozooni Institute of Immunology and Infectious Diseases, Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Mehrdad Haghighi Infectious Disease and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Shahrzad Soleimani Department of Molecular Genetics, Institute of Basic Science, Shahrekord Islamic Azad University, Iran
  • Rezvan Ghadyani Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Ehsan Parsazad Department of Bioscience and Biotechnology, Malek Ashtar University, Tehran, Iran
  • Sepideh Azari Department of Molecular Cell Biology, Faculty of Biological Science, Islamic Azad University Varamin Pishva Branch, Tehran, Iran
  • Mostafa Rezaei Tavirani Proteomics Research Center, system Biology Institute, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran Iran
  • Sepehr Kahrizi School of Medicine, Islamic Azad University, Qeshm Branch, Qeshm, Iran
  • Ehsan Rouhollahpour Ahangar Department of Prosthodontics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Abolfazl Movafagh Men's Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Keywords:

Colorectal cancer (CRC), SMAD4, Diagnostic biomarkers, Real-Time PCR (qRT-PCR)

Abstract

Colorectal cancer (CRC) ranks as the third leading cause of cancer-related deaths globally. SMAD4 gene acts as the central mediator of the signaling pathway for transforming growth factor-β (TGFβ) with a significant effect on colorectal cancer. Previous research has confirmed a relationship between the presence of the SMAD4 gene and the survival and progression of colorectal cancer in patients. In this study, our goal was to analyze the presence of SMAD4 in both colorectal cancer and nearby normal tissues. The expression levels of SMAD4 were evaluated in 45 colorectal tumor tissues and 45 adjacent control tissues using the Quantitative Real-Time PCR (qRT-PCR) method. Additionally, we assessed the diagnostic effectiveness of SMAD4 by creating a receiver operating characteristic (ROC) curve. Our findings showed that the expression of SMAD4 was significantly reduced in colorectal cancer patients compared to the adjacent control group sample. Examination of clinicopathological characteristics of patients revealed varied correlations between SMAD4 gene expressions and TMN stage (p<0.0001). These findings suggest that SMAD4 levels could be used as possible diagnostic indicators for colorectal cancer.

Published

2024-10-31

Issue

Vol. 70 No. 9: Issue 9

Section

Original Research Articles

License

Copyright (c) 2024 Neda Mansouri, Zahra Mozooni, Mehrdad Haghighi, Shahrzad Soleimani, Rezvan Ghadyani, Ehsan Parsazad, Sepideh Azari, Mostafa Rezaei Tavirani, Sepehr Kahrizi, Ehsan Rouhollahpour Ahangar, Abolfazl Movafagh

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.